Vector Lab代理VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

AK-6000

SKU Unit Size Price Qty
AK-6000 1 kit

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Description
Specifications
Kit Components
Documents
Related Products
Citations
Technical Information

Description

The VECTASTAIN?ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN?ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

Features:

  • Uses biotin/streptavidin technology
  • Can detect as little as 1 pg target protein when used with DuoLuX Substrate
  • Standard Kit contains Reagent A and Reagent B only
  • Sufficient reagents to develop twenty 100 cm2 blots

Specifications

More Information
Unit Size 1 kit
Applications Blotting Applications, Elispot
Technology ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s) Alkaline Phosphatase
Target Species No antibody included
Format Concentrate

Kit Components

Kit Contents:

  • 0.5 ml Reagent A and 0.5 ml Reagent B

Documents

  • User Guide
  • Troubleshooting Guide for VECTASTAIN ABC-AmP Kits
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

ImmPACT? Vector? Red Substrate, Alkaline Phosphatase (AP) Catalog #: SK-5105

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Vector? Black Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5200

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Vector? Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

DuoLuX? Chemiluminescent and Fluorescent Substrate, Alkaline Phosphatase (AP) Catalog #: SK-6605-100

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

10x Casein Solution Catalog #: SP-5020-250

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated Catalog #: BA-1000-1.5

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Horse Anti-Mouse IgG Antibody (H+L), Biotinylated Catalog #: BA-2000-1.5

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

DuoLuX? Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) Catalog #: SK-6604-200

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) Catalog #: SK-5400

Citations

VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection) Powered by Bioz See more details on Bioz

Technical Information

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

Home All Products VECTASTAIN ABC (Avidin-Biotin Complex Kits) VECTASTAIN ABC Kits for Western Blot Detection VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)

Vector Lab代理VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

PK-4004

SKU Unit Size Price Qty
PK-4004 1 kit

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Description
Specifications
Kit Components
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

The VECTASTAIN?ABC Reagent can be used to detect any biotinylated molecule. This is the original avidin/biotin ABC complex formation with biotinylated anti-rat?IgG secondary antibody.

Features:

  • Good sensitivity
  • Low background
  • Kit contains sufficient reagents to stain approximately 1000-2000 sections

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Blotting Applications, Elispot, ELISAs
Technology ABC (Avidin/Biotin) Systems
Detection Enzyme(s) Peroxidase
Target Species Rat
Format Concentrate

Kit Components

Kit Contents:

  • 2 ml Reagent A
  • 2 ml Reagent B
  • 1 ml biotinylated rabbit anti-rat IgG
  • 3 ml normal rabbit serum

Documents

  • Avidin/Biotin Troubleshooting Guide
  • User Guide
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

How do I choose which VECTASTAIN ABC Kit to use?

We offer a choice of either peroxidase or alkaline phosphatase based VECTASTAIN ABC kit detection systems. Selection of which enzyme system to use would be the first step. Most IHC applications involve detecting an unconjugated primary antibody. Consider the species in which the primary antibody is raised (e.g. rabbit), and then match to to corresponding species-specific VECTASTAIN ABC kit (e.g. VECTASTAIN ABC Rabbit IgG kit). If you already have a biotinylated target (i.e. biotinylated secondary antibody or primary antibody), then a standard VECTASTAIN ABC kit containing just the avidin/biotinylated enzyme reagents would be required. Note that only the VECTASTAIN Universal Elite PLUS ABC kit (PK-8200) contains a substrate.

Should the VECTASTAIN ABC kit only be stored at 4 degrees C? Can it be stored at -20 degrees C?

We do not recommend freezing the ABC kits. In particular, the ABC reagents exhibit a decrease in enzyme activity and lose some sensitivity when stored at – 20 °C for extended periods.

Related Products

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

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VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

VECTASTAIN? ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Rabbit Anti-Rat IgG Antibody (H+L), Biotinylated Catalog #: BA-4000-1.5

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Rabbit Anti-Rat IgG Antibody, mouse adsorbed (H+L), Biotinylated Catalog #: BA-4001-.5

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Normal Rabbit Serum Blocking Solution Catalog #: S-5000-20

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

ImmPACT? DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

ImmPACT? NovaRED? Substrate, Peroxidase (HRP) Catalog #: SK-4805

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ImmPACT? VIP Substrate, Peroxidase (HRP) Catalog #: SK-4605

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

ImmPACT? SG Substrate, Peroxidase (HRP) Catalog #: SK-4705

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

ImmPACT? AMEC Red Substrate, Peroxidase (HRP) Catalog #: SK-4285

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

ImmPACT? AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Vector? VIP Substrate Kit, Peroxidase (HRP) Catalog #: SK-4600

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Vector? SG Substrate Kit, Peroxidase (HRP) Catalog #: SK-4700

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Vector? NovaRED? Substrate Kit, Peroxidase (HRP) Catalog #: SK-4800

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine) Catalog #: SK-4100

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

TMB Substrate Kit, Peroxidase (HRP), (3,3', 5,5'-tetramethylbenzidine) Catalog #: SK-4400

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

AEC Substrate Kit, Peroxidase (HRP), (3-amino-9-ethylcarbazole) Catalog #: SK-4200

Citations

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG) Powered by Bioz See more details on Bioz

Technical Information

With more than 50,000 citations to its credit, the VECTASTAIN?ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN?ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

Kit Contents:

  • 3 ml normal rabbit serum
  • 1 ml biotinylated rabbit anti-rat IgG secondary antibody
  • 2 ml each of concentrated A and B reagents

Product review on Biocompare.com.

The Avidin Biotin Complex Method

The VECTASTAIN?ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.

The VECTASTAIN?ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:

  • Tissue staining
  • Multiple labeling (Multiplex IHC)
  • Western blotting
  • Southern and northern blotting
  • In situ hybridization detection (ISH)
  • Enzyme immunoassays (ELISA)
  • Neuronal tracing

All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN?ABC system.

VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

?

Choosing a VECTASTAIN?ABC Kit

1) Choose an enzyme system:

The VECTASTAIN?ABC Kits are available with a choice of two different enzyme systems

  • Peroxidase (ABC, Elite??ABC, RTU or Kits)
  • Alkaline Phosphatase (ABC-AP)

2) Choose the biotinylated secondary antibody:

To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate.

To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN?ABC Kit designated “Rabbit IgG”.

For additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (which is included in our VECTASTAIN?Universal Kits) is a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody should not be used to stain rat, mouse, or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.

3) Choose a convenient format:

Finally, several kits are available in prediluted, stabilized formats for convenience and ease of use. See kits designated R.T.U. or Ready-To-Use.

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the biotinylated secondary antibody may cross react with endogenous IgG in the tissue section leading to background staining. The following three options minimize background staining in these instances:

  • Biotinylate the primary antibody and detect with a Standard VECTASTAIN?Elite?ABC Kit, PK-6100 (only includes the avidin-biotin complex, or ABC reagent).
  • Use a biotinylated secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
  • Use the M.O.M.? Immunodetection System for applications of mouse primary antibodies on mouse tissue.

Substrates

After choosing the VECTASTAIN?ABC Kit for your application, select a substrate that matches the enzyme system of the kit.

Home All Products VECTASTAIN ABC (Avidin-Biotin Complex Kits) VECTASTAIN ABC Kits (HRP) VECTASTAIN Original ABC Kits (Peroxidase) VECTASTAIN? ABC-HRP Kit, Peroxidase (Rat IgG)

Vector Lab代理VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

PK-7800

SKU Unit Size Price Qty
PK-7800 50 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Description
Specifications
Kit Components
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

The VECTASTAIN Universal Quick HRP Kit can detect any biotinylated molecule.?Staining can be completed?in less than 20 minutes following primary antibody incubation.

Features:

  • Rapid protocol:?Working solutions can be used immediately after dilution.
  • High sensitivity, low background
  • Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*
  • Available in Ready-To-Use (R.T.U) format: Prediluted, stabilized working solutions of VECTASTAIN Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN Universal Kit.

*Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.

?

Sufficient reagents to stain approximately 250-500 sections.

Specifications

More Information
Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry
Technology ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s) Peroxidase
Target Species Universal (Mouse/Rabbit/Goat)
Format Ready-to-Use

Kit Components

Kit Contents:

Each of the stabilized, prediluted reagents are supplied in dropper bottles.

  • 50 ml 2.5% normal horse serum
  • 50 ml prediluted, biotinylated, universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
  • 50 ml prediluted peroxidase-streptavidin complex

Documents

  • Avidin/Biotin Troubleshooting Guide
  • User Guide
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

How do I choose which VECTASTAIN ABC Kit to use?

We offer a choice of either peroxidase or alkaline phosphatase based VECTASTAIN ABC kit detection systems. Selection of which enzyme system to use would be the first step. Most IHC applications involve detecting an unconjugated primary antibody. Consider the species in which the primary antibody is raised (e.g. rabbit), and then match to to corresponding species-specific VECTASTAIN ABC kit (e.g. VECTASTAIN ABC Rabbit IgG kit). If you already have a biotinylated target (i.e. biotinylated secondary antibody or primary antibody), then a standard VECTASTAIN ABC kit containing just the avidin/biotinylated enzyme reagents would be required. Note that only the VECTASTAIN Universal Elite PLUS ABC kit (PK-8200) contains a substrate.

Related Products

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Streptavidin/Biotin Blocking Kit Catalog #: SP-2002

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

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VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

ImmPACT? DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105

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ImmPACT? NovaRED? Substrate, Peroxidase (HRP) Catalog #: SK-4805

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

ImmPACT? VIP Substrate, Peroxidase (HRP) Catalog #: SK-4605

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

ImmPACT? SG Substrate, Peroxidase (HRP) Catalog #: SK-4705

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

ImmPACT? AMEC Red Substrate, Peroxidase (HRP) Catalog #: SK-4285

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

ImmPACT? AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Vector? VIP Substrate Kit, Peroxidase (HRP) Catalog #: SK-4600

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Vector? SG Substrate Kit, Peroxidase (HRP) Catalog #: SK-4700

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Vector? NovaRED? Substrate Kit, Peroxidase (HRP) Catalog #: SK-4800

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine) Catalog #: SK-4100

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

AEC Substrate Kit, Peroxidase (HRP), (3-amino-9-ethylcarbazole) Catalog #: SK-4200

Citations

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U. Powered by Bioz See more details on Bioz

Technical Information

With more than 50,000 citations to its credit, the VECTASTAIN?ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN?ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN?Universal Quick Kits

The VECTASTAIN?Universal Quick Kit is designed for rapid immunohistochemical staining of tissue sections. The kit relies on a proprietary preformed peroxidase-streptavidin complex to achieve outstanding sensitivity with short incubation times.

Kit Contents:

Each of the stabilized, prediluted reagents are supplied in dropper bottles.

  • 50 ml 2.5% normal horse serum
  • 50 ml prediluted, biotinylated, universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
  • 50 ml prediluted peroxidase-streptavidin complex

?

?

The Avidin Biotin Complex Method

The VECTASTAIN?ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.

The VECTASTAIN?ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:

  • Tissue staining
  • Multiple labeling (Multiplex IHC)
  • Western blotting
  • Southern and northern blotting
  • In situ hybridization detection (ISH)
  • Enzyme immunoassays (ELISA)
  • Neuronal tracing

All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN?ABC system.

VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

?

Choosing a VECTASTAIN?ABC Kit

1) Choose an enzyme system:

The VECTASTAIN?ABC Kits are available with a choice of two different enzyme systems

  • Peroxidase (ABC, Elite?ABC, RTU or Kits)
  • Alkaline Phosphatase (ABC-AP)

2) Choose the biotinylated secondary antibody:

To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate.

To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN?ABC Kit designated “Rabbit IgG”.

For additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (which is included in our VECTASTAIN?Universal Kits) is a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody should not be used to stain rat, mouse, or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.

3) Choose a convenient format:

Finally, several kits are available in prediluted, stabilized formats for convenience and ease of use. See kits designated R.T.U. or Ready-To-Use.

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the biotinylated secondary antibody may cross react with endogenous IgG in the tissue section leading to background staining. The following three options minimize background staining in these instances:

  • Biotinylate the primary antibody and detect with a Standard VECTASTAIN?Elite?ABC Kit, PK-6100 (only includes the avidin-biotin complex, or ABC reagent).
  • Use a biotinylated secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
  • Use the M.O.M.? Immunodetection System for applications of mouse primary antibodies on mouse tissue.

Substrates

After choosing the VECTASTAIN?ABC Kit for your application, select a substrate that matches the enzyme system of the kit.

Home All Products VECTASTAIN ABC (Avidin-Biotin Complex Kits) VECTASTAIN ABC Kits (HRP) VECTASTAIN Universal Quick Kits (Peroxidase) VECTASTAIN? Universal Quick HRP Kit, Peroxidase, R.T.U.

Vector Lab代理Goat Anti-Human IgG Antibody (H+L), Unconjugated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Goat Anti-Human IgG Antibody (H+L), Unconjugated

AI-3000-1.5

SKU Unit Size Price Qty
AI-3000-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Description
Specifications
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Technical Information

Description

Unconjugated Goat Anti-Human IgG Antibody?binds to human?primary antibodies for?immunohistochemistry and many?other applications.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Ready for iodination, fluorochrome labeling, or enzyme conjugations
  • Can be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage 2-8 °C; Store frozen for long term storage
Recommended Usage Reconstitute by adding 0.5 ml water. The resulting solution will have the following composition: 10 mM phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide.
Target Species Human
Conjugate Unconjugated
Host Species Goat
Format Lyophilized

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Goat Anti-Human IgG Antibody, gamma chain specific, Biotinylated Catalog #: BA-3080-.5

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Rabbit Anti-Goat IgG Antibody (H+L), Biotinylated Catalog #: BA-5000-1.5

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Goat Anti-Human IgG Antibody (H+L), Fluorescein Catalog #: FI-3000-1.5

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Goat Anti-Human IgG, gamma chain specific, Fluorescein Catalog #: FI-3080-.5

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Goat Anti-Human Kappa Chain Antibody, Biotinylated Catalog #: BA-3060-.5

Goat Anti-Human IgG Antibody (H+L), Unconjugated

Goat Anti-Human IgG Antibody (H+L), Biotinylated Catalog #: BA-3000-1.5

Citations

Goat Anti-Human IgG Antibody (H+L), Unconjugated Powered by Bioz See more details on Bioz

Technical Information

The goat anti-human Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.?

Unconjugated Goat Anti-Human IgG Antibody is supplied in carrier-free, lyophilized form. This ultrapure, high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination, fluorochrome labeling, or enzyme conjugations. It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins.

Home All Products Unconjugated Secondary Antibodies Goat Anti-Human IgG Antibody (H+L), Unconjugated

Vector Lab代理Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Goat Anti-Human Antibody, Kappa Chain, Unconjugated

AI-3060-.5

SKU Unit Size Price Qty
AI-3060-.5 0.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Description
Specifications
Documents
Related Products
Technical Information

Description

Unconjugated Goat Anti-Human Kappa Chain Antibody binds to human?primary antibodies for?immunohistochemistry and many?other applications.?The unlabeled form can be used as a capture antibody in enzyme immunoassays or radioactively labeled for radioimmunoassays.?This kappa?chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.

Specifications

More Information
Unit Size 0.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1 mg/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose
Target Species Human
Conjugate Unconjugated
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Goat Anti-Human IgG Antibody, Gamma Chain, Unconjugated Catalog #: AI-3080-.5

Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Goat Anti-Human Kappa Chain Antibody, Biotinylated Catalog #: BA-3060-.5

Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Goat Anti-Human IgM Antibody, Mu Chain, Unconjugated Catalog #: AI-3020-.5

Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Goat Anti-Human IgG Antibody (H+L), Unconjugated Catalog #: AI-3000-1.5

Technical Information

The goat anti-human Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.?

Unconjugated Goat Anti-Human Kappa Chain Antibody is supplied in solution. This ultrapure, high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination, fluorochrome labeling, or enzyme conjugations. It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins.

This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins. This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies.?

Home All Products Unconjugated Secondary Antibodies Goat Anti-Human Antibody, Kappa Chain, Unconjugated

Vector Lab代理Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

AI-9100-1.5

SKU Unit Size Price Qty
AI-9100-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

The?goat?anti-hamster IgG antibody recognizes IgG from both Syrian and Armenian hamsters. It can be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins.

Features:?

  • Recognizes both heavy and light chains (H+L)?
  • Ready for iodination, fluorochrome labeling, or enzyme conjugations?
  • Supplied in solution

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 3 mg/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose
Target Species Hamster
Conjugate Unconjugated
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated Catalog #: BA-9100-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Cat IgG Antibody (H+L), Biotinylated Catalog #: BA-9000-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Chicken IgY Antibody (H+L), Biotinylated Catalog #: BA-9010-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Guinea Pig IgG Antibody (H+L), Biotinylated Catalog #: BA-7000-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Horse IgG Antibody (H+L), Biotinylated Catalog #: BA-8000-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Human IgG Antibody (H+L), Unconjugated Catalog #: AI-3000-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Rabbit IgG Antibody (H+L), Unconjugated Catalog #: AI-1000-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Goat Anti-Mouse IgG Antibody (H+L), Unconjugated Catalog #: AI-9200-1.5

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Rabbit Anti-Goat IgG Antibody (H+L), Unconjugated Catalog #: AI-5000-1.5

Citations

Goat Anti-Hamster IgG Antibody (H+L), Unconjugated Powered by Bioz See more details on Bioz

Technical Information

The goat anti-hamster IgG antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.?

This ultrapure, high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination, fluorochrome labeling, or enzyme conjugations.?

Home All Products Unconjugated Secondary Antibodies Goat Anti-Hamster IgG Antibody (H+L), Unconjugated

Vector Lab代理Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

BA-1000-1.5

SKU Unit Size Price Qty
BA-1000-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

The biotinylated goat anti-rabbit IgG?secondary antibody is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques
  • Included in the VECTASTAIN??ABC kits

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 2-10 μg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This product is at the same concentration as that provided in the VECTASTAIN? ABC kits.
Target Species Rabbit
Conjugate Biotinylated
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Texas Red? Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

VECTASTAIN? ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

VECTASTAIN? ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 488 Catalog #: SA-5488-1

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 549 Catalog #: SA-5549-1

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 594 Catalog #: SA-5594-1

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 649 Catalog #: SA-5649-1

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

Citations

Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Biotinylated Goat Anti-Rabbit IgG (H+L) is supplied in liquid format. With some exceptions, the recommended dilution for most applications is 1:200.

Home All Products Biotinylated Secondary Antibodies Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated

Vector Lab代理Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

BA-1100-1.5

SKU Unit Size Price Qty
BA-1100-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

The biotinylated horse?anti-rabbit IgG?secondary antibody is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L)?
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
  • Supplied in solution?

?

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide. Immunohistochemical grade bovine serum albumin has been added for stabilization.*
Recommended Usage The recommended concentration range for use is 2-10 μg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This is not the same antibody as that which is provided in the VECTASTAIN? ABC kits.
Target Species Rabbit
Conjugate Biotinylated
Host Species Horse
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Texas Red? Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

VECTASTAIN? ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

VECTASTAIN? ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 488 Catalog #: SA-5488-1

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 549 Catalog #: SA-5549-1

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 594 Catalog #: SA-5594-1

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 649 Catalog #: SA-5649-1

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Citations

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.?
With some exceptions the recommended dilution for most applications is 1:200.

Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications. This antibody can be detected with the VECTASTAIN ABC systems or other (strept)avidin reagents.

Home All Products Biotinylated Secondary Antibodies Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated

Vector Lab代理Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated

BA-1400-2.1

SKU Unit Size Price Qty
BA-1400-2.1 2.1 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

This biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies.?This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Recognizes both heavy and light chains (H+L)?
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
  • Can be used for tissue and cell staining, ELISAs, and blots?
  • Included in the VECTASTAIN? ABC kits
  • Supplied as solution
  • Recommended dilution for most applications is 1:50
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

Note:?This product is the same biotinylated secondary antibody included in the VECTASTAIN??Elite ABC Kit (Universal; PK-6200) and VECTASTAIN??ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN?Universal Quick Kit, PK-8800.

Specifications

More Information
Unit Size 2.1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.05 mg/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 10-20 μg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This product is the same solution as that provided in the VECTASTAIN? Universal ABC Elite? kit (cat. no. PK-6200) and VECTASTAIN? Universal ABC-AP kit (cat. no. AK-5200).
Target Species Universal (Mouse/Rabbit)
Conjugate Biotinylated
Host Species Horse
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

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Citations

Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.

Home All Products Biotinylated Secondary Antibodies Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated

Vector Lab代理Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命,該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

BA-6000-1.5

SKU Unit Size Price Qty
BA-6000-1.5 1.5 mg

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Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

The biotinylated rabbit?anti-sheep?IgG?secondary antibody is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies?
  • Recognizes both heavy and light chains (H+L)?
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
  • Included in the VECTASTAIN? ABC kits?
  • Supplied in solution

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 2-10 μg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-sheep IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. Note:This product is at the same concentration as that provided in the VECTASTAIN? ABC kits.
Target Species Sheep
Conjugate Biotinylated
Host Species Rabbit
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

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Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

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Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

VECTASTAIN? ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

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VECTASTAIN? ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

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VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

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Streptavidin, DyLight? 488 Catalog #: SA-5488-1

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Streptavidin, DyLight? 549 Catalog #: SA-5549-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 594 Catalog #: SA-5594-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight? 649 Catalog #: SA-5649-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Citations

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.?

With some exceptions the recommended dilution for most applications is 1:200.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker? solution (Cat. No. SP-5030) is recommended.

Home All Products Biotinylated Secondary Antibodies Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated