AppliChem A7089.0500 核酸污染清除試劑 DNA-ExitusPlusl

AppliChem A7089.0500 核酸污染清除試劑 DNA-ExitusPlusl

DNA-ExitusPlus 核酸污染清除試劑 ( A7089,0500)
產品名稱:DNA-ExitusPlus 核酸污染清除試劑(500ml)
Product Name: DNA-ExitusPlus
品牌:PanReac Applichem
產品貨號: A7089,0500
生產廠家: AppliChem GmbH
原產國:德國

聚合酶鏈式反應(Polymerase Chain Reaction)簡稱PCR,是一種選擇性體外擴增核酸片段的方法,經過循環復制,擴增產量是指數上升,所以具有極高的靈敏度,被廣泛應用于生物和醫學的科研,臨床各種研究中,是目前應用最為廣泛的分子生物學方法之一。
但也正是由于PCR具有極高的靈敏度,微量的核酸污染便會導致假陽性擴增結果而導致實驗結果不準確,這也是廣大實驗者最頭痛的問題,如果形成氣溶膠污染擴散,則可引起整個PCR實驗室的污染,處理起來非常棘手,嚴重的甚至要關閉實驗室。環境中的核酸是主要的污染源,常見的污染源有:
1. 模板提取時真空抽干裝置
2. 超凈臺
3. 電泳裝置
4. 紫外分析儀
5. 剪刀、鑷子、刀片、移液器等器械
6. 離心機
7. 冰箱門把手,冷凍架,門把手或實驗臺面等
8. 氣溶膠
9. 高壓滅菌裝置

傳統的PCR污染清除方法主要有:稀酸處理法、紫外照射法、尿嘧啶糖苷酶(UNG)法、酶解變性、酒精沉淀等幾種方式,但仍然存在無法徹底破壞DNA分子、試劑具有腐蝕性或者毒性、小片段核酸無法清除等不足。

德國AppliChem獨家研發的DNA-ExitusPlus是一種無腐蝕、無致癌性的核酸污染清除溶液,該產品可將裸露的核酸完全降解至無法作為擴增模板的小片段,無法被擴增,且無法恢復,從而徹底消除了PCR過程中由于環境污染而導致的假陽性擴增。

DNA-ExitusPlusTM特點:
1. 產品為非酶類試劑;
2. 產品中各組分協同作用,快速、非序列特異性的降解核酸污染;
3. 所有組分可生物降解、無毒無害;
4. 不含有機溶劑或者揮發性物質,不含無機酸或者堿性物質,不會對金屬形成腐蝕;
5. 使用簡單,噴灑或浸泡處理15分鐘即可完成清除作用;
6. 升溫至50度可提高反應速度。

產品參數
性狀:無色或者淺黃色液體
保存溫度:常溫
運輸溫度:常溫
有效期:12個月

訂貨信息?(Ordering Information)

產品貨號:?A7089,0500? (500ml)

產品信息?

貨號 品名 用途 規格
A7089 DNA-ExitusPlusTM 環境中核酸污染清除 250ml/500ml/1L/2.5L
A7409 DNA-ExitusPlusTM?IF (indicator free) 250ml/500ml/1L/2.5L
A8740 Derma-ExitusPlusTM 處理手,手臂,手套等 250ml
A7600 Autoclave-ExitusPlus? 高壓滅菌裝置中核酸污染清除 6x 1 L

使用方法:
1. ?實驗操作臺表面
直接將DNA-ExitusPlusTM噴到操作臺表面作用10~15分鐘,無菌濕布擦拭后即可,無需用水沖洗;
2. ?儀器表面
用布或紙巾蘸取適量 DNAExitusPlusTM擦拭儀器表面,充分作用后再用干凈濕布擦一遍即可。一些小的部件可在DNA-ExitusPlusTM中浸泡10min左右,然后用清水沖洗、晾干;
3. ?塑料及玻璃器皿
向容器中加入足量的DNA-ExitusPlusTM,搖晃以確保器皿內壁全部接觸到溶液,充分作用后棄去溶液晾干,用蒸餾水沖洗干凈晾干即可;
4. ?移液器
遵照移液器說明拆取移液器的管嘴連件,于DNA-ExitusPlusTM中浸泡1min,然后用清水沖洗干凈晾干便可安裝到移液器上;
5. ?解剖刀、鑷子等實驗器材
先用蘸有DNA-ExitusPlusTM的布擦拭一遍,然后將鑷子等器材于DNA-ExitusPlusTM中浸泡10min以上,也可通過加熱到50~60℃縮短處理時間;
6. ?手臂、手套等
直接將Derma-ExitusPlus(A8740對準手臂或手套進行噴霧至全部濕潤,作用3~4min即可)。

此產品與方法已得到全球知名雜志與科研工作者的認可:
(1) Esser, K.-H. et al. (2006) DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents. Nature Methods 3, 151
(2) Arena, A. (2010) DNA exitus plus™ versus standard bleach solution for the removal of dna contaminants on work surfaces and tools. Investigative sciences journal 2, 20-29

AppliChem A2132 Aprotinin BioChemica CAS 9087-70-1

AppliChem A2132 Aprotinin BioChemica CAS 9087-70-1
? ? ? ? ?AppliChem品牌試劑代理-上海金畔生物科技有限公司
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AppliChem A2132 Aprotinin BioChemica CAS 9087-70-1

Physical Description:??Solid

Product Code:?A2132

Product Name:??Aprotinin BioChemica

Headline Comment:

? registered trademark of Bayer AG

Specifications:

Assay (protein): min. 90 %
Activity (KIU/mg): min. 5500
pH (1 %; H2O): 5.0 – 7.5
Water (K.F.): max. 6 %

Comment:

Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor?(BPTI) and Trypsin-kallikrein Inhibitor?(TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 – 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.Stability\: Aprotinin may be stored indefinite at \+4°C in lyophilized form. Dissolved in saline or buffer solutions (e. g. 10 mg/ml) at pH 5 – 8, it may be stored aliquoted at \+4°C at least for one month or frozen at -20°C for several years. Thawed aliquots of the stock solutions should not be frozen again, because repeated freeze-thawing will lead to aggregation of the polypeptide aprotinin. Solutions with a pH value below 4 or above 9 should be used immediately. Protect from direct sunlight, UV light and reducing agents. It is stable in water, 70 % methanol, 70 % ethanol or 50 % acetone, too (2, 3).Unit-Definition: One Trypsin Inhibitor Unit (TIU) will decrease the activity of 2 trypsin units by 50 %, where one trypsin unit will hydrolyze 1.0 μmole Nα-benzoyl-DL-arginine p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (Trypsin Inhibitor Unit) corresponds to approx. 1300 KIU (Kallikrein Inhibitor Units) and 1 TIU corresponds to one Ph. Eur. Unit.

Bibliography:

(1)Kassell, B. & Laskowski, Sr., M. (1965) Biochem. Biophys. Res. Com. 20,?463-468.The basic Trypsin inhibitor of bovine pancreas\: V. The disulfide linkages. (2)Kassell, B. (1970) Methods Enzymol. 19, 844-852Bovine Trypsin-Kallikrein inhibitor (Kunitz Inhibitor, Basic Pancreatic Trypsin Inhibitor, Polyvalent Inhibitor from Bovine Organs). (3)Fritz, H. & Wunderer, G. (1983) Drug Res. 33, 479-494Biochemistry and application of Aprotinin, the Kallikrein inhibitor from bovine organs.

Storage:??2-8°C

產品名稱:Aprotinin BioChemica
規格:10mg
貨號:A2132_0010
廠商:AppliChem
產品介紹:
Storage: 2-8°C
LGK: 10 – 13
Formula C284H432N84O79S7
M 6511.52 g/mol
CAS-No.: 9087-70-1
HS-No.: 35040090
origin from bovine lung
Specification
Assay (Protein) min. 90 %
Activity (KIU/mg) min. 5500
pH (1 %; H2O) 5.0 – 7.5
Water (K.F.) max. 6 %
Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor (BPTI) and Trypsin-kallikrein Inhibitor (TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 – 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.
AppliChem? A2132_0010?Aprotinin BioChemica?10mg?543
AppliChem? A2132_0025?Aprotinin BioChemica?25mg?? 1117
AppliChem? A2132_0100?Aprotinin BioChemica?100mg? 3936

AppliChem Myco系列支原體污染清除試劑

AppliChem Myco系列支原體污染清除試劑

AppliChem品牌試劑代理-上海金畔生物科技有限公司
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Myco-1|A5222_0010?AppliChem

產品名稱:Myco-1
規格:10ml
貨號:A5222_0010
廠商:AppliChem
產品介紹:
Storage: -20°C
Shipment: wet ice
Synonym Tiamulin
usage sterile solution
HS-No.: 38220000
for the treatment of Mycoplasma-infected cells.
Application in combination with Myco-2 (A5233).
100X concentrated antibiotic solution.
based on the antibiotic Tiamulin
AppliChem?A5222_0010?Myco-1?10ml?929
AppliChem?A5222_0020?Myco-1?20ml?1614

Myco-2|A5233_0010?AppliChem

產品名稱:Myco-2
規格:10ml
貨號:A5233_0010
廠商:AppliChem
產品介紹:
Synonym Minocycline
usage sterile solution
HS-No.: 38220000
Storage: -20°C
Shipment: wet ice
for the treatment of Mycoplasma-infected cells.
Application in combination with Myco-1 (A5222).
100X concentrated antibiotic solution.
based on the antibiotic Minocycline
The contamination of cells with mycoplasma is a very common problem, even though it often goes unnoticed since no cloudiness appears in the cell culture. Nevertheless the contamination often causes biochemical changes as well as changes in the immunological properties of the cells. Since mycoplasma-infected cells cannot always be discarded, many complicated methods have been suggested for the elimination of the mycoplasma. AppliChem offers a combination of antibiotics, which have been shown to be effective in the elimination of mycoplasma species that account for 90 % of the contamination found in cell cultures.
AppliChem?A5233_0010?Myco-2?10ml?731
AppliChem?A5233_0020?Myco-2?20ml?1104

Myco-3|A5240_0010?AppliChem

產品名稱:Myco-3
規格:10ml
貨號:A5240_0010
廠商:AppliChem
產品介紹:
Lagerung: -20°C
HS-Nr.: 38220000
für die Behandlung Mycoplasmen-infizierter Zellen.
Anwendung ohne zus?tzliche Antibiotika.
100X konzentrierte Antibiotika-L?sung.
basiert auf dem Antibiotikum Ciprofloxacin
AppliChem?A5240_0010?Myco-3?10ml?514
AppliChem?A5240_0020?Myco-3?20ml?929
AppliChem?A5240_0100?Myco-3?100ml?3737

德國 Applichem A7089 DNA-ExitusPlus 核酸污染清除試劑

德國 Applichem A7089 DNA-ExitusPlus 核酸污染清除試劑

AppliChem品牌試劑代理-上海金畔生物科技有限公司
? ? ? ?上海金畔生物代理AppliChem品牌試劑,歡迎新老客戶訪問AppliChem官網或者咨詢我們獲取相關產品信息。
? ? ? 德國艾普力(APPLICHEM)代理

Product Code:??A7089

Product Name:?DNA-ExitusPlus™

Specifications:

  • removes DNA and RNA contaminations from surfaces
    ? for cleaning of PCR work stations and equipment
    ? for cleaning of electrophoresis equipment, pipetts, reaction tubes etc.
    ? delivered as spray bottles or as refill bottles (RF)
    ♦ The special features
    ? non-enzymatic degradation of DNA and RNA by catalytic and cooperative effects of the components
    ? All components of DNA-ExitusPlus™ are readily biologically degradable and not harmful or toxic for humans.
    ? Doesn’t contain aggressive mineralic acids or alkaline substances
    ? No toxic fumes. Contains low concentration of alcohol.
    Functionality (Strand breakage test): passes test
    Efficacy (ExitusPlus™ activity test): passes test

Comment:

DNA amplification is one of the most commonly used techniques in the modern research laboratory. The presence of contaminating DNA in and around PCR workstations can lead to unwanted artifacts during amplification. Principally, there are two ways to make DNA not amplifiable:1. by degradation of the DNA (e. g. by the addition of DNases or chemical destruction), or2. by modification of bases – leaving the DNA strand intact, but blocked for reading by polymerases. Using a DNA strand break assay (designed by multiBIND biotec GmbH, Germany), it has been shown that not all DNA decontamination solutions on the market totally degrade DNA! DNA-ExitusPlus™ is an improvement over those products and causes both strand breakages as well as degradation. When used properly at the work area, it totally eliminates the amplification of non-target DNA. DNA-ExitusPlus™ is a non-alkaline, non-corrosive and non-carcinogenic cleansing solution which is effective on all surfaces.A severe disadvantage of conventional decontamination reagents is revealed in a new test for the corrosive potential of their components. For this purpose, different metal plates were incubated for 20 minutes with identical aliquots of the reagents. The selected metals are representative for equipment and materials found in laboratories. The result of this corrosion test show that all known commercial products contain aggressive chemical substances with corrosive, harmful or even toxic effects. These conventional reagents are known to contain azides, mineralic acids like phosphoric acid or hydrochloric acid, aggressive peroxides or strong alkaline substances like sodium hydroxide. Even after an incubation of only 20 minutes, irreversible damages of the metal surfaces are observed in many cases. The newly developed solution DNA-ExitusPlus™ exhibits its unique characteristics especially in this corrosion test. For all metal surfaces tested, no damage or corrosion is observed after treatment. DNA-ExitusPlus™ was also tested under identical conditions on many different plastic surfaces without any indications of damage.DNA-ExitusPlus™ is a registered trademark of AppliChem GmbH.

Bibliography:

(1)Esser, K.-H. et al. (2006) Nature Methods 3(2), 151DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents.

Storage:?RT???protected from light

Nature Methods推薦PCR污染清除方法——德國PanReac AppliChem DNA-ExitusPlus
貨號Cat.No.: A7089,0100(100ml)?? ??A7089,0500(500ml)?? ? ?A7089,1000(1L)
聚合酶鏈式反應(Polymerase Chain Reaction)簡稱PCR,是一種選擇性體外擴增核酸片段的方法,經過循環復制,擴增產量是指數上升,所以具有極高的靈敏度,被廣泛應用于生物和醫學的科研,臨床各種研究中,是目前應用最為廣泛的分子生物學方法之一。
但也正是由于PCR具有極高的靈敏度,微量的核酸污染便會導致假陽性擴增結果而導致實驗結果不準確,這也是廣大實驗者最頭痛的問題,如果形成氣溶膠污染擴散,則可引起整個PCR實驗室的污染,處理起來非常棘手,嚴重的甚至要關閉實驗室。環境中的核酸是主要的污染源,常見的污染源有:
1. 模板提取時真空抽干裝置
2. 超凈臺
3. 電泳裝置
4. 紫外分析儀
5. 剪刀、鑷子、刀片、移液器等器械
6. 離心機
7. 冰箱門把手,冷凍架,門把手或實驗臺面等
8. 氣溶膠
9. 高壓滅菌裝置

傳統的PCR污染清除方法主要有:稀酸處理法、紫外照射法、尿嘧啶糖苷酶(UNG)法、酶解變性、酒精沉淀等幾種方式,但仍然存在無法徹底破壞DNA分子、試劑具有腐蝕性或者毒性、小片段核酸無法清除等不足。

德國AppliChem獨家研發的DNA-ExitusPlus是一種無腐蝕、無致癌性的核酸污染清除溶液,該產品可將裸露的核酸完全降解至無法作為擴增模板的小片段,無法被擴增,且無法恢復,從而徹底消除了PCR過程中由于環境污染而導致的假陽性擴增。

DNA-ExitusPlusTM特點:
1. 產品為非酶類試劑;
2. 產品中各組分協同作用,快速、非序列特異性的降解核酸污染;
3. 所有組分可生物降解、無毒無害;
4. 不含有機溶劑或者揮發性物質,不含無機酸或者堿性物質,不會對金屬形成腐蝕;
5. 使用簡單,噴灑或浸泡處理15分鐘即可完成清除作用;
6. 升溫至50度可提高反應速度。

AppliChem A5219二氧化碳培養箱水源消毒劑 Incuwater-Clean

AppliChem A5219二氧化碳培養箱水源消毒劑 Incuwater-Clean

AppliChem品牌試劑代理-上海金畔生物科技有限公司
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AppliChem A5219二氧化碳培養箱水源消毒劑 Incuwater-Clean

Product Code:?A5219

Product Name:??Incuwater-Clean™

Headline Comment:

  • Incuwater-Clean™ is a registered trademark of AppliChem GmbH.

Specifications:

  • Disinfectant solution for CO2-incubator water baths.
    ? 100X concentrated solution. Use 50 ml per 5 liter of incubator water bath.
    ? for the prevention of microbial growth in incubator water baths.

Comment:

The water required to create the humidity is a source of contamination which disperses in the incubator. In order to disinfect the water we recommend Incuwater-Clean™ solution, which contains a disinfectant that does not cause damage to the stainless steel tray, is non-toxic, non-volatile, and extremely effective. The water should be replaced with sterile water every two to four weeks, adding 50 ml of Incuwater-Clean™ per 5 liters of water. This product can be applied in normal water baths too.

Storage:?2-8°C

【詳細說明】

實驗室里,細胞培養接觸水源是支原體,細菌,真菌和病毒zui容易傳播和交叉污染的地方,也是實驗室消毒zui難處理的地方,包括細胞培養箱產生的氣霧、培養箱內專用水源以及水浴鍋等設備,易污染、難清除、危害大等問題是威脅細胞正常生長和表達的重要因素。

來自德國素有”小Sigma”之稱的AppliChem,獨創Myco支原體清除系列,能有效的清除細胞培養過程中的支原體污染,為廣大科研工作者解決了困擾許久的難題,同時,也推出系列預防產品,以防止失而復得的細胞再次遭受污染之災。德國AppliChem獨創專門針對水池抗支原體污染的系列產品。產品應用于容器表面的消毒, 以及二氧化碳培養箱蓄水槽, 各種水箱, 及清洗用水池的消毒。

Incuwater-Clean 主要用于細胞培養箱內的水源消毒,為100X濃縮液,直接加入滅菌水中,使用方便,該試劑高效無毒,無揮發性,并且不會腐蝕金屬表面。建議培養箱中每2-4周更換一次滅菌水,并加入Incuwater-Clean。

訂購信息:

品牌 產品貨號 品名 規格
AppliChem A5219 Incuwater-Clean 100ml

另外還有用于CO2培養箱消毒劑Incubator-Clean噴霧消毒劑和水浴鍋消毒劑Aquabator-Clean,消毒徹底,安全無毒,使用方便,這一系列預防污染產品全方位為您的細胞保駕護航。

AppliChem A1117 BCIP BioChemica CAS:6578-06-9

AppliChem A1117 BCIP BioChemica CAS:6578-06-9

AppliChem品牌試劑代理-上海金畔生物科技有限公司
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? ? ? 德國艾普力(APPLICHEM)代理

Code:?A1117
CAS?:6578-06-9
Molecular Formula?:?C8H6BrClNO4P · C7H9N
Molar mass :433.64 g/mol
中文名稱:5-溴-4-氯-3-吲哚磷酸
英文名稱:BCIP BioChemica
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
廠商:AppliChem
產品介紹:
Storage: -20°C? protected from light
Solubility (20°C) 20 mg/ml (DMF)
Formula C8H6BrClNO4P · C7H9N
M 433.64 g/mol
CAS-No.: 6578-06-9
HS-No.: 29339980
EC-No.: 229-506-1
Specification
λmax. (buffer pH 7.0) 286 – 294 nm
E 1 %,1 cm, λmax. min. 5800 (pH 7.0)
Water (K.F.) max. 1 %
Synonym 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, X-Phos p-toluidine salt, X-Phos-pT
AppliChem?A1117_0001?BCIP BioChemica?1g?1929
AppliChem?A1117_0005?BCIP BioChemica?5g?7515
AppliChem?A1117_0250?BCIP BioChemica?250mg?660
AppliChem?A1117_0500?BCIP BioChemica?500mg?1119

德國AppliChem A3778 DNase I 脫氧核糖–上海金畔生物代理

德國AppliChem A3778 DNase I 脫氧核糖–上海金畔生物代理

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DNase I,即Deoxyribonuclease I,中文名稱為脫氧核糖核酸酶I,是一種可以消化單鏈或雙鏈DNA產生單脫氧核苷酸或單鏈或雙鏈的寡脫氧核苷酸的核酸內切酶。DNase I水解單鏈或雙鏈DNA后的產物,5’端為磷酸基團,3’端為羥基。
DNase I活性依賴于鈣離子,并能被鎂離子或二價錳離子激活。鎂離子存在條件下,DNase I可隨機剪切雙鏈DNA的任意位點;二價錳離子存在條件下,DNase I可在同一位點剪切DNA雙鏈,形成平末端,或1-2個核苷酸突出的粘末端。

Physical Description:? ?Solid

Product Code:? A3778

Product Name:? ?DNase I

Short Description:? ?delivery form: salt-free, freeze-dried powder, chromatographically prepared

Specifications:

Activity (Kunitz): min. 3000 U/mg

Comment:

Deoxyribonuclease I (DNase I) from beef pancreas is an endonuclease (glycoprotein), which preferentially cleaves the phosphodiester bond in the DNA behind pyrimidine nucleotides. This results in a polynucleotide with a 5′-phosphate and a free OH-group in position 3′. DNase I cleaves single-stranded and double-stranded DNA as well as chromatin. The specificity of the enzyme reaction (single-strand-‘Nicks’ versus double-strand breakes) is determined by the ions available. In the presence of Mg2\+?single-strand nicks are generated and in the presence of Mn2\+?double-strand breakes. The pH-optimum of DNase I is 7.8 and it is activated by divalent cations. Maximum activation requires the presence of Mg2\+?and additional Ca2\+. Calcium ions (5 mM) protect DNase I from proteolytic digest. Inhibition is achieved by citrate, if activation is done by magnesium, but not if manganese has been the activator. Besides it is inhibited by chelators such as EDTA and SDS or β-mercaptoethanol.The enzyme is used in molecular biology techniques like digestion of DNA, in the RNA purification (ref. 2 Suppl. 1 pp. 4.1.4) or generating “random nicks” for “nick translation” (ref. 2 Suppl. 9 pp. 3.5.4-6) or ‘footprint’-assays (ref. 2 Suppl. 7 chapter 12.4) or investigations on chromatin (ref. 2 Suppl. 48 chapter 21.4.1).Unit definition\: One unit is defined as that amount of enzyme which causes an increase of absorbance at 260 nm of 0.001 per minute at 25°C based on the method of Kunitz. DNase I is readily soluble in e. g. 0.15 M sodium chloride or in reaction buffer (e. g. 50 mM Tris · Cl, pH 7.5; 10 mM MgCl2?(single-strand ‘nicks’) and 10 mM MnCl2?(double-strand breakes), respecitvely; 50 μg/ml BSA; ref. 2 Suppl. 8 page 3.12.5). For storage dissolve DNase I in 50 % glycerol (w/v); 20 mM Tris · Cl, pH 7.5; 1 mM MgCl2. For stability reasons the concentrations should be at least 1 mg/ml (The maximum solubility is 10 %). This solution is stable for more than one year (ref. 2 Suppl. 8 page 3.12.5). The lyophilized form is stable for 2 – 5 years if kept at \+4°C. If a solution is protease-free, DNase I will not loose significant activity at pH 5 – 7 and 62°C for 5 hours. The enzyme may be heat-inactivated (10 minutes at 99°C).RNase-free DNase I: Dissolve DNase I at 1 mg/ml in 0.1 M iodoacetic acid plus 0.15 M sodium acetate at a final pH of 5.3. The solution is then heated 40 minutes at 55°C and cooled. Finally, 1 M CaCl2?is added to the solution to 5 mM. Store frozen in small aliquots (according to ref. 2 page 3.12.6 Supplement 8).

Bibliography:

(1)Sambrook, J. & Russell, D.W. (2001) Molecular Cloning\: A Laboratory Manual, 3rd Edition. pp. A4.40-42. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2001. Currrent Protocols in Molecular Biology. John Wiley & Sons, N.Y. (3)McDonald, M.R. (1955) Methods Enzymol. 2, 437-447Deoxyribonucleases (4)Campbell, V.W. & Jackson, D.A. (1980) J. Biol. Chem. 255, 3726-3735The effect of bivalent cations on the mechanism of DNase I. (5)Meinkoth, J. & Wahl, G.M. (1987) Methods Enzymol. 152, 91-94Nick-Translation. (6)Cobianchi, F. & Wilson, S.H. (1987) Methods Enzymol. 152, 94-110Enzymes for modifying and labeling of DNA and RNA.

Storage: -20°C

Origin: from bovine pancreas

產品信息:

品牌 貨號 品名 規格
AppliChem A3778,0050 DNase I 50mg
AppliChem A3778,0100 DNase I 100mg
AppliChem A3778,0500 DNase I 500mg
AppliChem A3778_0010 DNase I 10mg

保存溫度:-20℃,室溫運輸。

應用:DNase I 是一種需二價陽離子的脫氧核糖核酸內切酶,可用于降解單鏈或雙鏈DNA

用于無DNA污染的RNA的制備,逆轉錄及體外轉錄等實驗。

產品信息:M=~31000g/mol;CAS-NO:9003-98-9

性狀:凍干粉

配置方法:溶解在150 mM 5毫克/毫升生理鹽水,工作液為無色透明狀。

特點:不含RNase(RNase?free),可以用于各種RNA樣品的處理。
用途:制備不含DNA的RNA樣品;RT-PCR反應前RNA樣品中去除基因組DNA等可能的DNA污 染;體外T7, T3, SP6等RNA Polymerases催化的RNA轉錄后去除DNA模板; DNase I footprinting研究DNA-蛋白質相互作用;缺口平移(nick?translatioin);產生DNA隨機片斷文庫;細胞凋亡TUNEL檢 測中部分剪切基因組DNA作為陽性對照。
來源:牛胰腺純化得到。
分子量:約31kDa。
活性檢測條件:40mM Tris-HCl(pH8.0),10mM MgSO4,1mM CaCl2,1μg of pBR322 DNA。
純度:不含其它DNA內切酶和外切酶,不含RNA酶。
酶儲存溶液:50mM Tris-acetate(pH7.5),10mM CaCl2,50%(v/v)glycerol。
Reaction Buffer(10X)100mM Tris-HCl(pH7.5 at 25℃),25mM MgCl2,1mM CaCl2。
失活或抑制:加入EDTA至終濃度為2.5mM后,65℃加熱10分鐘可使DNase I失活。酚氯仿抽提也可以使DNase?I失活。金屬離子螯合劑,達到毫摩爾/升濃度的鋅離子,0.1%的SDS,DTT、巰基乙醇等還原 劑,50-100mM以上鹽濃度均對DNase I有顯著抑制作用。

注意事項:
如需對酶進行稀釋,可以用酶儲存液(50mM?Tris-acetate(pH?7.5),10mM?CaCl2,50%(v/v)glycerol)進行稀釋。
酶使用時宜存放在冰盒內或冰浴上,使用完畢后宜立即放置于-20℃保存。
為了您的安全和健康,請穿實驗服并戴一次性手套操作。

Applichem A3744 支原體PCR檢測試劑盒 PCR Mycoplasma Test Kit

Applichem A3744 支原體PCR檢測試劑盒 PCR Mycoplasma Test Kit

AppliChem品牌試劑代理-上海金畔生物科技有限公司
? ? ? ?上海金畔生物代理AppliChem品牌試劑,歡迎新老客戶訪問AppliChem官網或者咨詢我們獲取相關產品信息。
? ? ? 德國艾普力(APPLICHEM)代理

【簡單介紹】
AppliChem推出的PCR Mycoplasma Kit(貨號:A3744)是一個即用型支原體檢測試劑盒,無需支原體培養,只需要從培養細胞液中取上清,離心后獲得可能含有支原體的沉淀,用50ul Kit內提供的緩沖液重懸后,95℃熱擊3分鐘后即可作為PCR模板,加入Kit內提供的PCR反應液,并配成50ul PCR反應體系后即可按照Protocal提供的PCR設置進行PCR反應(如左圖所示)。同時該支原體檢測Kit內提供陽性對照,可完成20次檢測。

Product Code:? A3744

Product Name:? PCR Mycoplasma Test Kit

Specifications:

♦ Ready-to-use PCR Mix for the detection of mycoplasma in cell culture
♦ detects all of myoplasma species found in cell cultures
♦ for 20 tests
♦ Kit components
? Reaction mix
? Buffer solution
? Positive template control
? Internal control DNA template
? Internal control primers mix

Comment:

The PCR Mycoplasma Test Kit?is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. Mycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labeled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready-to-use, optimized PCR mix is supplied. The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum, M. capricolum) as well as Acholeplasma?and Spiroplasma?species, with high sensitivity and specificity.Principle\: rRNA gene sequences of prokaryotes, including mycoplasmas, are well conserved, whereas, the lengths and sequences of the spacer region in the rRNA operon (for example the region between 16S and 23S gene) differ from species to species. The detection procedure utilizing the PCR process with this primer set consists of1.) Amplification of a conserved and mycoplasma-specific 16S rRNA gene region using two primers.2.) Detection of the amplified fragment by agarose gel electrophoresis.This system does not allow the amplification of DNA originating from other sources, such as cultured cells or bacteria, which affect the detection result. Amplification of the gene sequence with PCR using this primer set enhances not only the sensitivity, but also the specificity of detection. Amplified products are then detected by agarose gel electrophoresis.

Bibliography:

(1)Rottem, S. & Barile, F.M. (1993) TIBTECH 11, 143-150

Storage:? -20°C
Avoid repeated changes in the reaction mix temperature
When in use, always keep the reaction mix on ice!

Shipment:? ?wet ice in Germany, dry ice to abroad

【詳細說明】

針對支原體檢測,有多種方案可供選擇,包括直接培養法、DNA熒光染色法等。其實就支原體污染的檢測,zui簡單有效的方案不外乎是使用PCR方法檢測。即以支原體16S-23S之間極為保守的rRNA序列設計引物,經由PCR反應擴增其16S rRNA片段。該法非常靈敏(e.g. 0.1-1.6 CFU/5 ul 樣品) 。由于引物的特殊性,不會導致培養細胞或其他細菌的序列的假性擴增。方法便捷,只需不到一天時間,并且無需支原體或細胞的培養過程,避免了可能的污染。

供應AppliChem A3672 DMSO 二甲基亞砜

供應AppliChem A3672 DMSO 二甲基亞砜

AppliChem品牌試劑代理-上海金畔生物科技有限公司

上海金畔生物代理AppliChem品牌試劑,歡迎新老客戶訪問AppliChem官網或者咨詢我們獲取相關產品信息。

德國艾普力(APPLICHEM)代理

供應AppliChem A3672 DMSO 二甲基亞砜

供應AppliChem A3672 DMSO 二甲基亞砜

CAS:67-68-5

Molecular Formula :C2H6OS

Molar mass:78.13 g/mol

Product Code:? A3672

Product Name:? ?Dimethyl sulfoxide Cell culture grade

Specifications:

Pyrogen test: passes test
Assay (GC): min. 99.5 %
Free acid: max. 0.001 %
Non-volatile matter: max. 0.001 %
Total P: max. 0.00001 %
Total Si: max. 0.00002 %
Water (K.F.): max. 0.1 %
Ca: max. 0.0001 %
Cu: max. 0.00005 %
Fe: max. 0.0001 %
K: max. 0.00005 %
Mg: max. 0.00005 %
Na: max. 0.0002 %
Pb: max. 0.00002 %
Zn: max. 0.00002 %

Comment:

Freezing of cells for cell culture \:Dimethyl sulfoxide (DMSO) is widely used for the freezing of cells in cell culture instead of glycerol. DMSO is added to prevent the formation of ice crystals during the freezing process, otherwise cells would be destroyed. DMSO is commonly used at the concentration of 10%. Please note: DMSO is toxic to cells! Therefore it is advisable to use pre-chilled “freeze medium”. Usually, such freeze medium consists of standard culture medium supplemented with 10% DMSO. Some researchers use 90% serum supplemented with 10% DMSO as freeze medium, which enhances the viability in some cell types. Particular cell types cannot be transferred into liquid nitrogen directly, but are slowly frozen to -70°C in a refrigerator. For very slow cooling of these stocks, cells are frozen well insulated and subsequently transferred to liquid nitrogen. Thawing: For further cultivation thawing should be carried out as quickly as possible. After thawing we recommend to wash the cells in medium or to change the medium the day after plating.

Applichem支原體預防試劑–A9390 Aquabator-Clean

Applichem支原體預防–A9390 Aquabator-Clean

AppliChem品牌試劑代理-上海金畔生物科技有限公司
? ? ? ?上海金畔生物代理AppliChem品牌試劑,歡迎新老客戶訪問AppliChem官網或者咨詢我們獲取相關產品信息。
? ? ? 德國艾普力(APPLICHEM)代理

支原體感染發生后能改變細胞的DNA,RNA及蛋白表達,又不能通過可視法對其進行檢測,而且它對細胞的生長率影響較小,不易引起注意。實驗室里,細胞培養接觸水源是支原體最容易傳播和交叉污染的地方,也是實驗室消毒最難處理的地方,這里包括細胞培養箱產生的氣霧、培養箱內專用水源以及水浴鍋等設備。德國的AppliChem公司獨創了專業針對水源抗支原體污染的系列產品,用于容器表面的消毒, 以及二氧化碳培養箱蓄水槽, 各種水箱, 及清洗用水池的消毒,下面詳細介紹一下細胞培養箱水源消毒劑—Aquabater-Clean(a9390)。

Aquabater-Clean可用于預防各種水浴鍋中真菌、細菌污染,是一種新型的即用型的方便快捷100倍濃縮液,它的有效成分對人是安全的,當在推薦的濃度下使用時(使用時每升水中加10ml?Aquabater-Clean即可)對人的皮膚不會引起任何的刺激,而且該試劑高效無毒,安全可降解,2-8°C避光儲存。

Applichem支原體預防–A9390 Aquabator-Clean

Product Code:
A9390
Product Name:
Aquabator-Clean™ (100X)
Headline Comment:
? Aquabator-Clean™ is a registered trademark of AppliChem GmbH.
Specifications:
? Disinfectant solution for ordinary water baths (not for CO2-incubators!)
? for the prevention of microbial growth in water baths
? 100X concentrated solution (Use 10 ml per 1 liter of water.)
品牌 貨號 品名 規格
AppliChem A9390 Aquabator-Clean ?(100X) 250ml

AppliChem為我們的客戶連續不斷的提供了可靠的和質量保證,產品都是經過國際ISO 9001認證的, 而且能夠滿足廣大用戶的滿足和特殊要求。另外,AppliChem還提了供針對細胞培養過程中各種可能支原體的污染源進行消毒的產品,如CO2培養箱的噴霧消毒Incubator-Clean™ (A5230)—一種即用型培養箱噴霧消毒劑,能有效預防真菌(及孢子)、細菌(及桿菌孢子)、支原體以及病毒污染(包括 HIV和乙肝病毒),它的有效成分是一組混合液,安全高效,不包含甲醛、苯酚等有害物質,是生物可降解的,建議每一周噴一次;培養箱內的水源消毒溶液Incuwater-Clean™ (A5219)—用于細胞培養箱內的水源消毒,該支原體預防試劑為100X濃縮液,直接稀釋進水源使用,能有效殺滅并預防水源內的支原體污染,該試劑高效無毒,無揮發性,并且不會腐蝕金屬表面,使用時用無菌水稀釋100倍,每2-4周更換新液。