AAT-Texas Red-streptavidin conjugate

AAT-Texas Red-streptavidin conjugate
Upgrade:?iFluor™ 594-streptavidin conjugate
See also:?Bioconjugation,?Biotin and Streptavidin,?Flow Cytometry Reagents
Molecular weight
52000
Correction Factor (260?nm)
0.23
Correction Factor (280?nm)
0.36
Correction Factor (565?nm)
0.4472
Extinction coefficient (cm?-1?M?-1)
1160001
Excitation (nm)
586
Emission (nm)
603
Quantum yield
0.931,?0.352
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Texas Red-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Texas Red covalently attached (as the fluorescent label). Our Texas Red-streptavidin conjugate was prepared using AAT Bioquest’s proprietary labeling technology. It demonstrated much brighter signal compared to the similar Texas Red-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Texas Red filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Calculators

Common stock solution preparation

Table 1.?Volume of?Water?needed to reconstitute specific mass of?Texas Red?-streptavidin conjugate?to given concentration. Note that volume is?only?for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 1.923 μL 9.615 μL 19.231 μL 96.154 μL 192.308 μL
5 mM 384.615 nL 1.923 μL 3.846 μL 19.231 μL 38.462 μL
10 mM 192.308 nL 961.538 nL 1.923 μL 9.615 μL 19.231 μL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.
鏈霉親和素綴合物與生物素綴合物一起廣泛用于特異性檢測各種蛋白質、蛋白質基序、核酸和其他分子,因為鏈霉親和素對生物素具有非常高的結合親和力。該德克薩斯紅-鏈霉抗生物素偶聯物包含鏈霉抗生物素(作為生物素結合蛋白)與德克薩斯紅共價連接(作為熒光標記)。我們的 Texas Red-streptavidin 偶聯物是使用 AAT Bioquest 的專有標記技術制備的。與來自其他商業來源的類似德州紅-鏈霉親和素偶聯物相比,它顯示出更亮的信號,因此可以顯著提高檢測靈敏度。當與生物素化一抗結合使用時,它通常用作間接免疫熒光染色的第二步試劑。它是使用流式細胞儀和 Texas Red 過濾器組進行基于生物素-鏈霉親和素的生物測定和測試的寶貴工具。多種互補的生物素化試劑可從眾多商業供應商處獲得。

普通儲備溶液制備

表 1.將特定質量的Texas Red?-鏈霉親和素偶聯物重新配制到給定濃度所需的水體積。請注意,體積用于制備儲備溶液。有關適當的實驗/生理緩沖液,請參閱示例實驗協議。
0.1毫克 0.5毫克 1毫克 5毫克 10毫克
1毫米 1.923 μL 9.615 μL 19.231 μL 96.154 μL 192.308 μL
5毫米 384.615 毫升 1.923 μL 3.846 μL 19.231 μL 38.462 μL
10毫米 192.308 毫升 961.538 毫升 1.923 μL 9.615 μL 19.231 μL

摩爾濃度計算器

輸入任意兩個值(質量、體積、濃度)來計算第三個。

Spectrum

光譜

Spectral properties?光譜特性

Correction Factor (260?nm) 0.23
Correction Factor (280?nm) 0.36
Correction Factor (565?nm) 0.4472
Extinction coefficient (cm?-1?M?-1) 1160001
Excitation (nm) 586
Emission (nm) 603
Quantum yield 0.931,?0.352

Citations

View all?1 citations:?Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors:?Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal:?Oncology letters?(2015):?1989–1996

References 參考文獻

View all?47 references:?Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors:?Largy E, Hamon F, Teulade-Fichou MP.
Journal:?Methods.?(2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors:?Mittal R, Bruchez MP.
Journal:?Bioconjug Chem?(2011):?362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors:?Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal:?J Fluoresc?(2011):?647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors:?Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal:?BMC Biochem?(2010):?50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors:?Sun M, Du L, Gao S, Bao Y, Wang S.
Journal:?Steroids?(2010):?400
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors:?Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal:?Bioconjug Chem?(2010):?1385
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors:?Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal:?Chemphyschem?(2009):?1517
Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors:?Luschtinetz F, Dosche C, Kumke MU.
Journal:?Bioconjug Chem?(2009):?576
Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors:?Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal:?Nanotechnology?(2008):?235103
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors:?Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal:?Methods Mol Biol?(2008):?73

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